The deduced NSHC sequence encodes isoforms of 474 and 594aa, corresponding to proteins of 52 and 64kD, respectively.
Sequence analysis determined that NSHC shares an overall homology of 54% with SHC and approximately 70% homology in the PTB and SH2 domains.
Northern blot analysis detected a 10-kb NSHC transcript exclusively in brain, whereas SHC was expressed as 3.8-, 3.4-, and 2.8-kb transcripts in all tissues except brain.
Within the central nervous system, NSHC was highly expressed in cerebral cortex, hippocampus, amygdala, and caudate nucleus, with low-level expression in subthalamic nucleus, thalamus, putamen, corpus callosum, hypothalamus, medulla, substantia nigra, and cerebellum.
Expression of NSHC was undetectable in the spinal cord.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SHC3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val188-Gln433 of human SHC3, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
