84-4436-57　［受注停止］SHC1 (SHC-Transforming Protein 1, SHC, SHCA, p52SHC, p66, p66SHC, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) 200ul　365043

特徴

• The SHC gene encodes a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains.
• The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52kD, both containing a C-terminal SH2 domain.
• Adjacent to the SH2 region is a glycine- and proline-rich region.
• The 2 proteins differ in their N terminals.
• SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway.
• The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways, for example, facilitating the activation of RAS proteins in response to a variety of factors.
• SHC proteins are rapidly associated with and phosphorylated by growth factor receptors with intrinsic tyrosine kinase activity.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV GGDPEVRKQM PPPPPCPGRE LFDDPSYVNV QNLDKARQAV GGAGPPNPAI NGSAPRDLFD MKPFEDALRV PPPPQSVSMA EQLRGEPWFH GKLSRREAEA LLQLNGDFLV RESTTTPGQY VLTGLQSGQP KHLLLVDPEG VVRTKDHRFE SVSHLISYH
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.