SHBG is a glycoprotein that binds to sex hormones, specifically testosterone and estradiol.
Other steroid hormones such as progesterone, cortisol, and other corticosteroids are bound by transcortin.
These sex hormones circulate in the bloodstream, bound mostly to SHBG and to some degree bound to serum albumin.
Only a small fraction is unbound, or "free," and thus biologically active and able to enter a cell and activate its receptor.
The SHBG inhibits the function of these hormones.
Thus bioavailability of sex hormones is influenced by the level of SHBG.
SHBG is produced by the liver cells and is released into the bloodstream.
Other sites that produce SHBG are the brain, uterus, and placenta and vagina.
In addition SHBG is produced by the testes; testes-produced SHBG is also called androgen-binding protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat SHBG.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro44-Leu222 of rat SHBG, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
