Proline-rich peptide sequences have been shown to play important roles in protein-protein interactions that occur in signal transduction pathways.
Specific proline-rich consensus sequences that bind SH3 domains have been identified.
The predicted 114aa SH3BGRL protein has a potential cell attachment sequence.
A proline-rich sequence containing an SH3-binding motif is present in the middle of the protein.
The SH3BGRL protein shares 60% identity with the proline-rich central region of SH3BGR.
Northern blot analysis detected a single SH3BGRL transcript of approximately 1.9 kb that was present in all tissues examined.
Mouse cDNA encoding a deduced protein that is 95% identical to human SH3BGRL.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SH3BGRL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala114 of human SH3BGRL, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
