Sestrin-2 is a member of the sestrin family of PA26-related proteins.
The encoded protein may function in the regulation of cell growth and survival.
This protein may be involved in cellular response to different stress conditions.
The full-length SEST2 cDNA encodes a deduced 480aa protein predicted to be a compact globular domain protein composed predominantly of alpha-helical structures.
SEST2 has several potential serine-threonine and tyrosine phosphorylation sites, most of them located within the alpha helices.
Northern blot analysis revealed a 3.9-kb transcript expressed at low to moderate levels in most tissues examined.
In vitro translation of the cDNA resulted in a protein with an apparent molecular mass of about 60kD.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse SESN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser41-Thr480 of mouse SESN2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
