Semenogelin I is the predominant protein in semen.
The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa.
The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function.
The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely.
Two transcript variants encoding different isoforms have been found for this gene.
An open reading frame of 1,386 nucleotides encoded a signal peptide and a mature protein of 439aa residues, in which residues 85 to 136 were identical with a previously characterized semenogelin fragment.
An abundant seminal vesicular mRNA transcript of 1.8 kb codes for semenogelin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SEMG1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln24-Thr462 of human SEMG1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
