84-4436-37　SEMA7A (Semaphorin 7A, CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, SEMAK1, SEMAL, Semaphorin 7A, GPI Membrane Anchor, John Milton Hagen Blood Group, Semaphorin-K1, Semaphorin-L) 200ul　365023

特徴

• SEMA7A is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage.
• SEMA7A is also known as the John-Milton-Hagen (JMH) blood group antigen, an 80kD glycoprotein expressed on activated lymphocytes and erythrocytes.
• Czopik et al.
• (2006) found that T cells from immunized Sema7a -/- mice had increased proliferative responses to antigen that were not attributable to Sema7a deficiency on macrophages or dendritic cells.
• Sema7a -/- mice were prone to die at the onset of experimental autoimmune encephalomyelitis (EAE) and had higher clinical EAE scores compared with wildtype littermates.
• Delayed-type hypersensitivity responses were also enhanced in Sema7a -/- mice.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RVY GVFSNPWNYS AVCVYSLGDI DRVFRTSSLK GYHMGLPNPR PGMCLPKKQP IPTETFQVAD SHPEVAQRVE PMGPLKTPLF HSKYHYQKVV VHRMQASNGE TFHVLYLTTD RGTIHKVVES GDQDHSFVFN IMEIQPFHRA AAIQAISLDA DRRKLYVTSQ WEVSQVPLDM CEVYSGGCHG CLMSRDPYCG WDQDRCVSIY SSQRSVLQSI NPAEPHRECP NPKPDEAPLQ KVSLARNSRY YLTCPM
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.