CD100 was first identified as a cell surface protein of resting T cells; previous studies had shown that it was involved in lymphocyte activation.
Hall et al.
(1996) cloned CD100 from a cDNA library of phytohemagglutinin-activated human T cells.
Sequence analysis showed that CD100 encodes an 863aa polypeptide containing a secretory signal sequence, a single transmembrane domain, an immunoglobulin (Ig)-like domain, and a conserved 500aa sema domain.
Thus, Hall et al.
(1996) stated that CD100 is a member of the semaphorin family and the first semaphorin believed to be involved in the immune system.
See SEMA3Fand SEMA3B.
Northern blot analysis revealed that CD100 is expressed primarily as a 4.5-kb message in a variety of tissues, both hematopoietic and nonhematopoietic.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SEMA4D.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe502-Phe636 of human SEMA4D, fused to His-Tag at N-terminal expressed in E. coli.
