84-4436-32　SELP (Selectin, Platelet, CD62P, P-Selectin, PSEL, GMP140, LECAM3, GRMP, GMRP, PADGEM, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane) 200ul　365018

特徴

• P-selectin is the largest of the known selectins at 140kD.
• It contains nine consensus repeats (CR) and extends approximately 40nm from the endothelial surface.
• P-selectin is expressed in a-granules of activated platelets and granules of endothelial cells.
• Within minutes of stimulation of the endothelial cells by inflammatory mediators such as histamine, thrombin, or phorbol esters, P-selectin is surface-expressed.
• Expression of P-selectin also occurs from the surgical trauma endured during preparation of the tissues for intravital microscopy.
• The expression is short-lived, reaching its peak after only ten minutes.
• Additional synthesis of P-selectin is brought about within two hours by cytokines such as interleukin-1 (IL-1) or tumor necrosis factor a (TNF-a).
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- REC GELELPQHVL MNCSHPLGNF SFNSQCSFHC TDGYQVNGPS KLECLASGIW TNKPPQCLAA QCPPLKIPER GNMTCLHSAK AFQHQSSCSF SCEEGFALVG PEVVQCTASG VWTAPAPVCK AVQCQHLEAP SEGTMDCVHP LTAFAYGSSC KFECQPGYRV RGLDMLRCID SGHWSAPLPT CEAISCEPLE SPVHGSMDCS PSLRAFQYDT NCSFRCAEGF MLRGADIVRC DN
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.