L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes.
It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups.
It is cleaved by ADAM17.
The protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats.
L-selectin acts as a "homing receptor" for leukocytes to enter tissues at the site of inflammation.
Ligands present on endothelial cells will bind to leukocytes expressing L-selectin, causing them to become localised at that point.
The receptor is also found on the cell surfaces of "naive" T cells, which have not yet encountered their specific antigen.
This surface expression is lost after the cells are activated.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse SELL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val101-Thr343 of mouse SELL, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
