Sorbitol dehydrogenase is an enzyme in carbohydrate metabolism converting sorbitol, the sugar alcohol form of glucose, into fructose.
Together with aldose reductase, it provides a way for the body to produce fructose from glucose without using ATP.
Sorbitol dehydrogenase uses NAD+ as a cofactor; its reaction is sorbitol + NAD+ + H+ --> fructose + NADH.
A zinc ion is also involved in catalysis.
Organs that use it most frequently include the liver and seminal vesicle; it is found in all kinds of organisms from bacteria to humans.
A secondary use is the metabolism of dietary sorbitol, though sorbitol is known not to absorb as well in the intestine as its related compounds glucose and fructose, and is usually found in quite small amounts in the diet anyway (except when used as an artificial sweetener).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat SDH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala2-Pro357 of rat SDH, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
