SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12).SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene.
SDF-1beta is synthesized as a 93aa precursor that contains a 21aa signal sequence and a 72aa mature region.
The mature molecule exhibits a typical three antiparallel beta-strand chemokine-like fold.
There are no potential N-linked glycosylation sites.
N-terminal amino acid ’s 1-8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation.
The C-terminus is likely associated with heparin binding.
SDF-1beta circulates and undergoes proteolytic processing.
SDF-1beta are reported to be a monomers at neutral pH and physiologic ionic strength.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SDF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro23-Lys89 of human SDF1, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
