Scube3 targeted allele, which replaces the entire coding region for exons 2 and 3 with a neomycin-lacZ selectable marker cassette predicted to delete the first two EGF-like domains of the transcribed protein.
Scube3 embryos demonstrate strong beta-galactosidase activity in the early facial epithelium, including the branchial arches and facial processes, the otic vesicle, limb buds and neural tube.
In addition, strong reporter activity was identified in the epithelial compartments of developing teeth and hair follicles.
However, analysis of the Scube3 allele revealed that it encodes a truncated protein, which contains part of the spacer region and CUB domain.
It is likely that this protein retains functionality because analysis reveals that Scube3 mice are phenotypically normal.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SCUBE3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu807-Lys993 of human SCUBE3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
