The deduced human SCO2 protein of 266aa predicts an N-terminal mitochondrial targeting presequence of 41aa.
Human and yeast SCO polypeptides share the greatest identity in a core region, which lies between glycine-102 and glycine-242 in human SCO2.
In this region the amino acid identity between the 2 human proteins (54%) is less than that between the 2 yeast proteins (73%).
Moreover, human SCO2 and SCO1 show similar divergence from both yeast proteins suggesting that the 2 human genes are not orthologous to the 2 yeast genes, but rather are paralogous genes.
Northern blot analysis detected SCO2 expression as a 0.9-kb transcript in all 12 human tissues examined, with strongest signals in heart, skeletal muscle, brain, liver, and kidney.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.