Secretogranin III is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins.
Granins may serve as precursors for biologically active peptides.
Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown.
The deduced 468aa protein, which has a calculated molecular mass of 53kD, contains an N-terminal signal peptide, a secretogranin motif, 3 potential N-glycosylation sites, and a putative tyrosine sulfation site.
SCG3 shares 87%, 87%, and 56% overall identity with the mouse, rat, and Xenopus orthologs, respectively.
SCG3 localized to the perinuclear endoplasmic reticulum in transfected COS-7 cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SCG3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe20-Leu468 of human SCG3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
