SAT1 belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism.
It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells.
Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD).
All species below vertebrates had 1 copy of SSAT, whereas most all vertebrate species had functional SAT1 and SAT2 genes, with the exception of chicken and Xenopus.
Northern blot and EST analysis indicated that SAT1 was expressed at higher levels than SAT2 and in a wider range of tissues.
Whereas SAT1 showed a much greater preference for spermidine than spermine, SAT2 showed similar preference for the 2 polyamines.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat SAT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Glu171 of rat SAT1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
