S100 calcium binding protein A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer.
This contributes to creating an overall immunosuppressive microenvironemnt that may contribute to the inability of a protective or therapeutic cellular immune response to be generated by the tumor-bearing host.
Outside of malignancy, S100A9 in association with its dimerization partner, S100A8 (MRP8 or calgranulin A) signals for lymphocyte recruitment in sites of inflammation.
S100A9/A8 (synonyms: Calgranulin A/B; Calprotectin) are also regarded as marker proteins for a number of inflammatory diseases in humans, especially in rheumatoid arthritis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes bovine S100A9.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo
Immunogen：Recombinant protein corresponding to Ile12-Ala89 of bovine S100A9, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
