S100A14 is a member of a subfamily of proteins related by Ca(2+)-binding motifs to the EF-hand Ca(2+)-binding protein superfamily.
Using a proteomics approach to identify genes upregulated in breast cancer, followed by database analysis and PCR of a breast carcinoma cell line, Adam et al.
(2003) cloned S100A14, which they called BCMP84.
BCMP84 expression in normal tissue was restricted to stratified squamous epithelium of skin, cervix, and tonsil.
Weak staining was seen in normal breast ductal tissue, but strong immunoreactivity was detected in 10 of 58 (17%) breast cancer tissues.
BCMP84 was expressed in the plasma membrane of transiently transfected breast carcinoma cells.
By fluorescence in situ hybridization, Pietas et al.
(2002) mapped the S100A14 gene to chromosome 1q21 in a cluster with at least 15 other S100 genes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human S100A14.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala91 of human S100A14, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
