Using expressed sequence tag (EST) analysis of unamplified cDNA libraries generated from human retina, Wistow et al.
(2002) identified an abundant novel open reading frame that encoded a 229-residue protein with a predicted molecular mass of 24.6kD
The corresponding gene, which they named retbindin (RTBDN), shows modest sequence homology to riboflavin-binding proteins of egg-laying vertebrates, and more distant similarity to mammalian folate receptors.
Analysis of the 5-prime ends of multiple RTBDN clones identified splice variants that suggested the presence of multiple promoters.
Northern blot analysis of rhesus monkey tissues showed retina-specific expression of a 1.2-kb RTBDN transcript.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse RTBDN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to His32-Pro247 of mouse RTBDN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
