RSPO3 encodes a member of the thrombospondin type 1 repeat supergene family.
In addition, the protein contains a furin-like cysteine-rich region.
Furin-like repeat domains have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases.
The 272aa protein has an N-terminal cys-rich domain (CRD) and a central thrombospondin type-1 (THBS1) repeat (TSR) domain.
Northern blot analysis detected 3.0- and 4.0-kb transcripts in placenta, lung, and skeletal muscle.
RT-PCR revealed ubiquitous expression, with highest levels in placenta, small intestine, fetal thymus, and lymph node.
RSPO3 expression was detected in all tumor tissues examined except a lung carcinoma and an ovarian carcinoma.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RSPO3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln22-His272 of human RSPO3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
