This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes.
C-Ros Oncogene 1 is a type I integral membrane protein with tyrosine kinase activity.
The protein may function as a growth or differentiation factor receptor.
ROS1 encodes a protein tyrosine kinase with a potential transmembrane domain.
It arose from the human ROS1 gene by a rearrangement introduced during gene transfer, which deleted the putative extracellular domain of ROS1, leaving potential transmembrane and intracellular protein tyrosine kinase domains intact.It contains an intracellular domain typical of tyrosine protein kinases, a transmembrane domain, a very large extracellular domain that contains 31 potential N-linked glycosylation sites, and a putative N-terminal signal peptide.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ROS1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu1945-Phe2222 of human ROS1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
