ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
ROCK2, which is an isozyme of ROCK1, is a target for the small GTPase Rho
By screening human brain cDNAs for the potential to encode proteins larger than 50kD, Ishikawa et al.
(1998) identified a ROCK2 cDNA, which they called KIAA0619.
RT-PCR detected ROCK2 expression in all human tissues examined.
By PCR of human cDNA using oligonucleotides based on the sequence of a bovine ROCK2 cDNA, followed by screening of a human brain cDNA library, Takahashi et al.
(1999) isolated a human ROCK2 cDNA.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
