ROCK1, is a protein serine/threonine kinase, It is activated when bound to the GTP-bound form of Rho GTPase.
This protein is thus a downstream effector of Rho.
It phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Thus, ROCK1 contributes to actin-stability.In a study of the expression of ROCK1 during the cell cycle of the corneal epithelium, they found that levels of ROCK1 were significantly lower in the G1 phase than in the S and G0 phases.
Downregulation of ROCK1 during the G1 phase is due in part to the decreased levels of its mRNA.
The authors concluded that ROCK1 may have a role in the progression of the cell cycle in the corneal epithelial cells as they migrate centripetally from the limbus across the corneal surface.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
