Rnd1 is a small (~21kD) signaling G protein (more specifically a GTPase), and is a member of the Rnd subgroup of the Rho family of GTPases.
It contributes to regulating the organization of the actin cytoskeleton in response to extracellular growth factors (Nobes et al., 1998).By screening a human fetal brain cDNA library using bovine Rnd1 as probe, Nobes et al.
(1998) cloned RND1.
The deduced protein contains 232aa and has a calculated molecular mass of 26kD.
It contains 3 guanine-binding motifs, 2 loops and a threonine involved in phosphate binding, and 3 major residues that coordinate magnesium in the GTP-bound state.
However, RND1 lacks the residues important for the intrinsic GTPase activity of RAS .
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RND1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Cys229 of human RND1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
