RNASE11 belongs to the pancreatic ribonuclease family.
Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA.
Bovine pancreatic RNase A is one of the classic model systems of protein science
RNase A is a relatively small protein.
It can be characterized as a two-layer alpha +Beta protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate.
The first layer is composed of three alpha helices from the N-terminal half of the protein.
The second layer consist of three Beta-hairpins arranged in two Beta-sheets.
The hairpins 61-74 and 105-124 form a four-stranded, antiparallel Beta-sheet that lies on helix 3.
The longest Beta-hairpin 79-104 mates with a short Beta-strand to form a three-stranded, antiparallel Beta-sheet that lies on helix 2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RNASE11.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala18-Leu199 of human RNASE11, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
