Sequence analysis predicted that GAIP is a 24.6kD, hydrophilic protein that lacks a transmembrane domain and contains multiple potential phosphorylation sites.
GAIP shares approximately 31% aa identity with regulator of G protein signaling-1 (RGS1) and -2 (RGS2); the 125aa core domains of GAIP, RGS1, and RGS2 share approximately 64% homology
Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney.
Yeast 2-hybrid binding analyses showed that through its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RGS19.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala217 of human RGS19, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
