Regucalcinis a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney.
It may have an important role in calcium homeostasis.
Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation.
This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23.
Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein.
Regucalcin is a proposed name for a calcium-binding protein that was discovered in 1978.
Regucalcin differs from calmodulin and other Ca2+-related proteins as it does not contain an EF-hand motif of Ca2+-binding domain.
It may regulate the effect of Ca2+ on liver cell functions.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat RGN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Gly299 of rat RGN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
