84-4435-13　RGC32 (Response Gene To Complement 32, RGCC, C13orf15, Regulator of cell cycle RGCC) 200ul　364899

特徴

• RGC32is thought to regulate cell cycle progression.
• It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle.
• The encoded protein localizes to the cytoplasm during interphase and to centrosomes during mitosis.
• The protein forms a complex with polo-like kinase 1.
• The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein.
• In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression.
• Detected in brain, heart and liver.
• Highly expressed in liver, skeletal muscle, kidney and pancreas.
• Detected at lower levels in heart, brain and placenta.
• Detected in aorta endothelial cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control (Supplied):364899A: Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.