RING finger protein 39 lies within the major histocompatibility complex class I region on chromosome 6.
Studies of a similar rat protein suggest that this gene encodes a protein that plays a role in an early phase of synaptic plasticity.
Alternative splicing results in three transcript variants encoding different isoforms.
The deduced proteins have 421, 355, and 257aa, respectively.
HZFW1 and HZFW2 contain both the N-terminal RING finger C3HC4 motif and the C-terminal B30-2-like domain, while HZFW3 lacks the B30-2-like motif.
Human testis expressed all 3 variants.
The deduced translation start site of HZFW1 differs from that of Lirf in that HZFW1 has an extra N-terminal stretch; however, both proteins retain the N-terminal RING finger motif.
Fluorescence-tagged Lirf protein localized to the cytoplasm of COS-7 cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RFN39.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser420 from human RFN39, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
