Resistin, a novel adipose-derived protein, has been proposed to cause insulin-resistant states in obesity.
Resistin is produced by white and brown adipose tissues but has also has been identified in several other tissues, including the hypothalamus, pituitary and adrenal glands, pancreas, gastrointestinal tract, myocytes, spleen, white blood cells and plasma.
Resistin antagonizes insulin action, and is down regulated by rosiglitazone and peroxisome proliferator- activated receptor gamma agonists.
Resistin is elevated in patients with type 2 diabetes and may play a role in the vascular complications of this disorder.
Recently, resistin has been discussed controversially as a missing link between obesity and insulin resistance.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine RETN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Cys22-Pro109 of porcine RETN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
