Renin-binding protein, originally isolated from porcine kidney, is a proteinaceous renin inhibitor.
In inhibiting renin, the protein forms a complex with it, so-called high molecular weight renin.
The nucleotide sequences of cDNAs encoding the porcine, human, and rat renin-binding proteins, the predicted amino acid sequences consist of 402, 417, and 419aa, respectively.
Sequences of the three are highly homologous.
The leucine zipper motif, which is a key structure for the formation of a heterodimer or a homodimer of several proteins, was also conserved in each RENBP.
it spans about 10 kb and consists of 11 exons separated by 10 introns.
By polymerase chain reaction amplification of hybrid DNAs from human and hamster somatic cells, they demonstrated that the RENBP gene is located on the X chromosome.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RENBP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Gln254 of human RENBp, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
