The REG4 gene encodes a deduced 158aa protein with an N-terminal signal peptide of 22aa and a predicted molecular mass of 18.2kD.
REG4 maintains conservation of the 6 cysteine residues present in other known members of the REG family.
By Northern blot analysis of intestinal mucosa from 12 individuals with inflammatory bowel disease, Hartupee et al.
(2001) identified a 1.1-kb REG4 transcript in all samples.
Control specimens had low but detectable expression of REG4.
REG4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis.
RNA dot-blot analysis showed that most of the tissues expressing REG4 are in the gastrointestinal tract, i.e., colon, small intestine, stomach, and pancreas.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RKLRNW SHAELECQSY GNGSHLASVL NPKEASVISK YITGYQRSLP VWIGLHDPQK NASWQWIDGS TNQYRPWSPR TKSEARHCTE MNPKDKFLTW NKNGCTKRQH F
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat REG4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg45-Phe151 of rat REG4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
