REG3G encodes a deduced 175aa protein, with a predicted molecular mass of 16.5kD after cleavage of a putative 26aa N-terminal signal peptide.
Two REG3G splice forms, with identical coding sequences but different 5-prime UTRs, were identified.
The REG3G and REG3A proteins share 85% sequence homology.
Northern blot analysis and RT-PCR experiments showed that REG3G is strongly expressed in pancreas, moderately in testis, and weakly in heart, kidney, and placenta.
Further RT-PCR analysis of pancreas-derived cell lines showed that REG3G expression is limited to exocrine pancreas.
Using 3-dimensional protein structural modeling, they showed that although REG3G and REG1A share similar structural features, they display distinctive surface charge distributions.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.