REG3G encodes a deduced 175aa protein, with a predicted molecular mass of 16.5kD after cleavage of a putative 26aa N-terminal signal peptide.
Two REG3G splice forms, with identical coding sequences but different 5-prime UTRs, were identified.
The REG3G and REG3A proteins share 85% sequence homology.
Northern blot analysis and RT-PCR experiments showed that REG3G is strongly expressed in pancreas, moderately in testis, and weakly in heart, kidney, and placenta.
Further RT-PCR analysis of pancreas-derived cell lines showed that REG3G expression is limited to exocrine pancreas.
Using 3-dimensional protein structural modeling, they showed that although REG3G and REG1A share similar structural features, they display distinctive surface charge distributions.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human REG3g.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe87-Asp175 of human REG3g, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
