Regenerating islet-derived protein 3 alpha is a pancreatic secretory protein that may be involved in cell proliferation or differentiation.
It has similarity to the C-type lectin superfamily.
The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis.
The predicted protein had the same size as the rat protein and showed 71% aa identity, the 6 half-cysteines being in identical positions.
As in the rat, the protein was expressed at very low levels in control tissues and overexpressed during the acute phase of pancreatitis in humans.
The protein could be immunolocalized to the apical regions of acinar cells.
The protein was also quantifiable in serum with an elevation of levels in pancreatitis, suggesting its possible use as a biologic marker of the disease.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GEDSQK AVPSTRTSCP MGSKAYRSYC YTLVTTLKSW FQADLACQKR PSGHLVSILS GGEASFVSSL VTGRVNNNQD IWIWLHDPTM GQQPNGGGWE WSNSDVLNYL NWDGDPSSTV NR
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes rat REG3a.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to Gly25-Arg142 of rat REG3a, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.