Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane.
It is highly similar in sequence to both ezrin and moesin.
The radixin gene has been localized by fluorescence in situ hybridization to 11q23.
A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3.
Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses.
The major full-length protein contains 627aa forming 3 known functional domains: an N-terminal FERM domain that localizes the protein to the plasma membrane, a central helical alpha-domain, and a C-terminal actin-binding domain.
By immunohistochemical analysis of mouse inner ear.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RDX.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ile5-Met295 of human RDX, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
