Recoverin is a 23kD neuronal calcium-binding protein that is primarily detected in the photoreceptors of the eye.
It plays a key role in the inhibition of rhodopsin kinase, a molecule which regulates the phosphorylation of rhodopsin.
This ultimately controls the ability of the eye to adapt to, and recover from, exposure to the presence of light.
The human cDNA clone also showed homology to the calcium-binding chick cone protein, visinin.
Analysis of the gene indicated that it is present in single copy and is 9 to 10 kb long with at least 3 exons and 2 introns.
The recoverin gene has 3 exons.
All 3 EF-hand binding motifs are encoded in the first exon.
The arrangement of introns relative to EF-hand calcium binding motifs is unique within the superfamily of EF-hand proteins, suggesting that recoverin defines a new subgroup.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RCVRN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly2-Ala200 of human RCVRN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
