Using microarray analysis to identify potential p53 (TP53) target genes in human cell lines, Shu et al.
(2006) identified and cloned 2 variants of RBM38, which they called RNPC1a and RNPC1b.
The deduced proteins contain 239 and 121aa, respectively, and both contain an identical N-terminal canonical RNA recognition motif made up of 2 conserved submotifs, RNP1 and RNP2.
Immunofluorescence analysis localized both proteins in the perinuclear membrane and cytosol, with a larger proportion of RNPC1b localized to the cytosol than the nucleus.
Both RNPC1a and RNPC1b were also induced by several p63 (TP63) and p73 (TP73) isoforms.
RNPC1 expression was induced by DNA-damaging agents in cells expressing wildtype p53, but not in cells lacking p53 expression.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RBM38.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His25-Tyr207 of human RBM38, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
