RBM3 is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain.
Expression of this gene is induced by cold shock and low oxygen tension.
A pseudogene exists on chromosome 1.
Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
The gene gave rise to alternatively spliced transcripts in a variety of human tissues.
The longest open reading frame encodes a 157aa protein with a predicted molecular weight of 17kD.
Its putative RNA-binding domain most closely resembles that of 2 previously characterized human RNA-binding proteins, YRRM, the gene which has been implicated in azoospermia, and a glycoprotein identified as an autoantigen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RBM3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Asn157 of human RBM3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
