Rap guanine nucleotide exchange factor 1 is a human guanine nucleotide releasing protein for Ras protein.
It belongs to the adaptor-type Src homology (SH)2-containing molecules
Src homology 2 domains are globular protein modules present in a large variety of functionally distinct proteins.
They mediate binding events that control the activity and localization of many proteins involved in the transmission of signals from the cell surface to the nucleus
The mRNAs of these proteins are expressed ubiquitously in human adult and fetal tissues.
Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RAPGEF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys780-Asn1035 of human RAPGEF1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
