cerevisiae YPT1 and SEC4 genes encode Ras-related GTP-binding proteins involved in the regulation of secretion.
Mammalian cells express a large number of RAB proteins, GTP-binding proteins closely related to YPT1 and SEC4.
Except for the closely related RAB3A and RAB3B, the deduced human RAB proteins share 32 to 50% homology.
The predicted 214aa RAB5 protein is 31% and 38% identical to SEC4 and YPT1, respectively.
All 6 human RAB proteins tested bound GTP and exhibited GTPase activities in vitro.
Northern blot analysis revealed that RAB5 was expressed as 2.7- and 2.8-kb mRNAs in a human fibroblast cell line.
RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RAB5A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser214 of human RAB5A, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
