Using yeast 2-hybrid analysis with mouse Rab5 as bait, Masuda et al.
(2000) identified Rab37 from a mouse mast cell line.
The deduced Rab37 protein contains sequence motifs conserved in other low molecular mass GTPases.
It shares 74% and 47% aa sequence identity with rat RAB26 and mouse Rab8 (RAB8A).
Northern blot analysis showed expression of 2.3- and 1.2-kb Rab37 transcripts exclusively in the MC9 mast cell line.
Immunoprecipitation followed by Western blot analysis of a panel of mouse tissues showed that a 34kD band corresponding to the Rab37 protein is expressed exclusively in bone marrow mast cells.
Expression of GFP-tagged Rab37 in bone marrow mast cells showed that Rab37 localizes to secretory granules.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RAB37.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser19-Cys220 of human RAB37, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
