The deduced 550aa PRR3 protein has a calculated molecular mass of 55.4kD and contains an N-terminal hydrophobic leader sequence; an ectodomain containing a V-type Ig domain and 2 C-type Ig domains bracketed by 6 cysteine residues; a hydrophobic transmembrane region; and a long cytoplasmic sequence.
PRR3 also has 6 potential N-glycosylation sites.
Northern blot analysis revealed PRR3 expression predominantly in placenta and testis, with faint or no expression in other tissues.
A major transcript of 2.8 kb and minor transcripts of 9.5, 6.0, and 4.7 kb were detected in placenta.
PRR3 was also expressed in mammalian epithelial cell lines.
Flow cytometry showed expression of PRR3 at the surface of transfected COS-1 cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PVRL3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser90-Ala293 of mouse PVRL3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
