Prothymosin alpha is a protein encoded by the PTMA.
PTMA is a small, 12.4kD protein.
It is a aa109-111 long polypeptide as the precursor of thymosin alpha-1.
The encoded human PTMA protein is a highly acidic (54 residues out of 111) and shared over 90% sequence homology with rat PTMA.
The primary structure of PTMA is highly conserved and shows a number of important features.
The first 28aa of its sequence correspond to those of thymosin-alpha-1.
It has a central acidic region (residues 41-85) comprising of glutamic and aspartic residues.
Neither type of secondary structure has been detected.
Thymosin-alpha-1 appeared at positions 2-29 of the PTMA.
PTMA has been shown to serve essential biological functions.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PTMa.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser2-Asp111 of human PTMa, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli
