Tyrosine-protein kinase 6 is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues.
Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.
Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue.
The encoded protein has been shown to undergo autophosphorylation
The deduced 451aa polypeptide sequence was composed of 3 domains: an SH3 domain, an SH2 domain, and a catalytic domain.
The sequence of BRK, unlike that of SRC, does not include an N-terminal myristoylation domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PTK6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe191-Thr445 of human PTK6, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
