PTI is a member of an emerging class of intracellular serpins.On the basis of its interaction with thrombin, through which it was discovered, the inhibitor was operationally named the placental thrombin inhibitor (PTI).
aa sequence comparisons suggested that its reactive center is located at arg341 and cys342, that it lacks a classic N-terminal signal sequence, and that it has the highest degree of similarity to intracellular serine proteinase inhibitors (serpins), such as the human monocyte/neutrophil elastase inhibitor (ELANH2).
Analysis of human tissue mRNA indicated that PTI is expressed widely, with the highest levels in cardiac and skeletal muscle and placenta.Spi3, is developmentally regulated, and that, unlike the situation in the human, Spi3 is absent from the mouse placenta.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PTI.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Pro376 of human PTI, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
