Phenol sulfotransferase, or PST (EC 2.8.2.1), catalyzes the sulfate conjugation of catecholamines and of phenolic drugs.
PST is widely distributed in human and animal tissues that include the blood platelets.
Human platelet PST exists in at least a thermolabile, or monoamine- metabolizing (SULT1A3, or STM), form and a thermostable, or phenol-metabolizing, form.
Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al.
(1982), Price et al.
(1988), and Van Loon and Weinshilboum (1984).
Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Pro3-Phe285 of mouse PST, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
