An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
PSMA5 encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.
The cDNA encoded a 241aa polypeptide.
The calculated and observed molecular masses were 26kD and 29kD, respectively.
Northern blot analysis revealed an mRNA of 1.2 kb in both human placenta and HeLa cells.
The alpha subunits comprise the outer rings of the proteasome.
Some alpha subunits contain a functional nuclear localization signal; proteasomes are found in both the nuclear and cytoplasmic compartments of the cell.
Alpha subunits may constitute a physical barrier that limits access of cytosolic proteins into the inner proteolytic chamber.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PSMa5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ile241 of human PSMa5, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
