PSAT catalyzes the second step in the pathway, conversion of 3-phosphohydroxypyruvate into 3-phosphoserine.
The full-length PSAT-beta transcript encodes a deduced 370aa protein with a calculated molecular mass of 40kD.
PSAT-alpha lacks exon 8 and encodes a deduced 324aa protein with a calculated molecular mass of 35.2kD.
Compared with PSAT-beta, PSAT-alpha lacks 46aa.
Both proteins contain a conserved binding domain for the cofactor pyridoxal 5-prime-phosphate (vitamin B6).
PSAT-beta shares 92.4% aa similarity with its mouse homolog.
PSAT-beta orthologs were present in all species examined, including plants, insects, and bacteria.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.