Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D.
Saposin is an acronym for Sphingolipid Activator PrO[S]teINs.
Each domain of the precursor protein is approximately 80aa residues long with nearly identical placement of cysteine residues and glycosylation sites.
Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups.
The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.
Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human PSAP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly17-Asn524 of human PSAP, expressed in E. coli.
